If the test was stored in the refrigerator, allow all materials (including the test cassette and buffer) to reach room temperature (15–25°C / 59–77°F) before testing.

Prepare a clean and flat working surface.

Have all materials ready before starting the test.

Use the provided lancet to gently prick the cat’s ear or paw.

Collect one drop of blood using the provided pipette.

Add 1 drop of blood into the assay buffer vial and mix well to ensure the blood is fully diluted.

Add 2 drops of the diluted sample into the sample hole of the test cassette.

Wait 5–10 minutes before reading the result. Do not interpret results after 10 minutes, as they are considered invalid.

The presence of both the C band and the A band is visible, no matter if the A band is clear or vague.

The presence of both the C band and the B band is visible, no matter if the B band is clear or vague.

The presence of both the C band, A band and B band is visible, no matter if the A band or B band is clear or vague.

No colored band appears in the C zone, no matter whether the A band or the B band appears.