Text instruction

Lay out all the materials.

For this test you should collect morning urine (preferably midstream urine) from the mare at least 50 days after insemination.

Note: Midstream urine means collecting the sample after the mare has started urinating, rather than collecting the first urine released.

Use a large clean bucket or cup to collect the urine sample.

Transfer a small amount of urine to the provided cup.

Allow the sample to reach room temperature for 20–30 minutes before testing.

Collect urine from cup using a pipette.

Place the test device on a flat surface at room temperature.

Add 6 drops of urine to the sample well (S).

Wait for the color bands to appear.

Read the result within 10–20 minutes.

Results read after 20 minutes are considered invalid.

A faint C (control) line and a strong T (test) line appear.

No C (control) line appears, regardless of whether a T (test) line is visible.