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    Preparations - image 1
    1

    Preparations

    Lay out all the materials.

    For this test you should collect morning urine (preferably midstream urine) from the mare at least 50 days after insemination.

    Note: Midstream urine means collecting the sample after the mare has started urinating, rather than collecting the first urine released.

    Collect Sample - image 1
    2

    Collect Sample

    Use a large clean bucket or cup to collect the urine sample.

    Transfer a small amount of urine to the provided cup.

    Allow the sample to reach room temperature for 20–30 minutes before testing.

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    3

    Use Pipette

    Collect urine from cup using a pipette.

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    Apply To Cassette

    Place the test device on a flat surface at room temperature.

    Add 6 drops of urine to the sample well (S).

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    5

    Wait

    Wait for the color bands to appear.

    Read the result within 10–20 minutes.

    Results read after 20 minutes are considered invalid.

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    Pregnancy / Positive

    • A strong or faint C (control) line appears.

    • A faint T (test) line appears, or no T line appears.

    No Pregnancy / Negative - image 1
    7

    No Pregnancy / Negative

    A faint C (control) line and a strong T (test) line appear.

    Invalid - image 1
    8

    Invalid

    No C (control) line appears, regardless of whether a T (test) line is visible.