If the test was stored in the refrigerator, allow all materials (including the test cassette and buffer) to reach room temperature (15–25°C / 59–77°F) before testing.

Prepare a clean and flat working surface.

Have all materials ready before starting the test.

Use the provided lancet to gently prick the dog's ear or paw.

Collect one drop of blood using the provided pipette.

Add 1 drop of blood into the assay buffer vial and mix well to ensure the blood is fully diluted.

Add 4 drops of the diluted sample into each sample hole of the test.

Wait 5–10 minutes before reading the result. Do not interpret results after 10 minutes, as they are considered invalid.

Both the C band and the T band are visible, regardless of whether the T band is strong or faint.

Only a clear C band is visible.

No colored C band appears, regardless of whether the T band is visible.

A retest is recommended.