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    Video instruction

    Text instruction

    Sample Collection - image 1
    1

    Sample Collection

    Masturbate and collect semen directly into the provided collection cup .

    Shake Jar - image 1
    2

    Shake Jar

    Shake the jar with semen evenly in the semen collection cup and leave it to stand for 1 hour at room temperature until the semen liquefies.
    Transfer Sample - image 1
    3

    Transfer Sample

    Use the provided transfer device/syringe to collect 0.1 mL of semen, marked on the device.

    Add To Vial - image 1
    4

    Add To Vial

    Add this to the vial containing the dilution buffer.

    Mix - image 1
    5

    Mix

    Mix the semen sample and test solution by turning the vial upside down 5-10 times.
    Prepare for Testing - image 1
    6

    Prepare for Testing

    Hold the vial upright and open the cap.

    Apply to Test Cassette - image 1
    7

    Apply to Test Cassette

    Add 2 full drops (~80 μL) of the diluted sample into the specimen well (S) of the test cassette. Start the timer immediately.

    Read results at 5 minutes, after 10 minutes results are no longer valid.

    Positive Results / Normal Levels - image 1
    8

    Positive Results / Normal Levels

    Two colored lines appear.

    One colored line should be in the control line region (C) and another colored line should be in the test line region (T).

    Note: The intensity of the color in the test line region (T) will vary depending on the concentration of SP-10 protein present in the specimen. Therefore, any shade of color in the test line region (T) should be considered normal.

    Negative Results - image 1
    9

    Negative Results

    One colored line appears in the control line region (C). No line appears in the test line region (T).

    A negative result indicates that results are abnormal, or is present below the detectable level of the test.

    Invalid Results - image 1
    10

    Invalid Results

    Control line fails to appear.

    Test did not work. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure.

    Review the procedure and repeat the test with a new cassette. If the problem persists, discontinue using the test kit and contact our support team.